中国水稻科学

中国水稻科学 ›› 2013, Vol. 27 ›› Issue (2): 137-144.DOI: 10.3969/j.issn.10017216.2013.02.005

• 研究报告 • 上一篇    下一篇

云南元阳哈尼梯田水稻地方品种月亮谷的遗传变异分析

董超1,# ,徐福荣1,# ,杨文毅1 ,汤翠凤1 ,张恩来1 ,杨雅云1 ,阿新祥1 ,张斐斐1,卢光德2,王艳2 ,戴陆园1,*   

  1. 1云南省农业科学院 生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业部西南作物基因资源与种质创制重点实验室/农业部云南稻种资源科学观测实验站，云南 昆明 650223；2 云南省元阳县农业局，云南 元阳 662414；
  • 收稿日期:2012-05-02 修回日期:2012-08-21 出版日期:2013-03-10 发布日期:2013-03-10
  • 通讯作者: 戴陆园1,*
  • 基金资助:

    国家863计划资助项目（2010AA101805）；云南省科技创新强省计划资助项目(2007C0219Z)；云南省社会发展科技计划项目(2010CC009; 2012CH009)；云南省人才培养资助项目(2008PY049)。

Genetic Variation Analysis of Paddy Rice Landrace of  Yuelianggu from Yuanyang Hani’s Terraced Fields in Yunnan Province

DONG Chao 1,# , XU Furong 1,# , YANG Wenyi 1, TANG Cuifeng 1, ZHANG Enlai 1 , YANG Yayun 1 , A Xinxiang 1 , ZHANG Feifei 1 , LU Guangde 2, WANG Yan 2, DAI  Luyuan 1, *   

  1. 1 Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences /Yunnan Provincial Key Laboratory  of Agricultural Biotechnology/ Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture/ Scientific Observation for Rice Germplasm Resources of Yunnan, Ministry of Agriculture, Kunming 650223, China; 2 Yuanyang Department of Agriculture,Yuanyang 662414, China;
  • Received:2012-05-02 Revised:2012-08-21 Online:2013-03-10 Published:2013-03-10
  • Contact: DAI Luyuan1, *

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被引次数

36

摘要： 为了揭示云南元阳哈尼梯田持续大面积种植的水稻地方品种月亮谷的遗传变异，利用20个表型性状和48个SSR标记，对采自元阳哈尼梯田11个村寨的98份月亮谷和8份对照品种进行分析。结果显示，98份月亮谷的质量性状变异系数较小；数量性状变异系数较大，变异系数范围为2.55%~24.90%；48个标记共检测出114个等位变异，平均为2.38个，变幅为1~4个；有效等位变异为87.65个，平均为1.83个，变幅为1~3.02；Nei遗传多样性（I）指数平均为0.64。表型聚类将月亮谷与对照品种分成两大类，所有月亮谷为一类，8个对照品种为另一类；SSR聚类则将所有月亮谷与4份籼型对照品种聚为一大类，4份粳型对照品种单独为一大类；分子方差分析（AMOVA）表明，月亮谷村寨之间变异（67.18%）大于村寨内（37.82%）。11个村寨月亮谷可以分成5个居群，这5个类群与其村寨间地理位置的距离相关，相邻村寨之间月亮谷遗传距离小，遗传相似度高。月亮谷这种特殊的遗传变异是由于独特的哈尼梯田及其传统文化长期作用的结果，月亮谷可作为优异基因资源进一步发掘和利用。

关键词: 哈尼梯田, 水稻, 遗传变异, 月亮谷

Abstract: In order to reveal the genetic variation of rice landrace Yuelianggu which has been widely grown in Yunnan Hani’s terraced fields for longterm,98 Yuelianggu samples  collected from 11 villages located in Yunnan and 8 rice varieties as control were analyzed by using 20 phenotypic traits and 48 SSR markers. The results showed that the variation coefficients for  the quantitative  characters   were larger than those of  the  qualitative characters  with a  range   from 2.55% to 24.90%.  A total of 114 alleles  in all of Yuelianggu were found with the mean of 2.38 and range from 1 to 4, as well as the number of 87.65 effective alleles with the mean of 1.83 and range from 1 to 3.02. The mean of Nei’s genetic diversity index (I) was 0.64. Clustering analysis based on phenotypes could divide the used materials including Yuelianggu and  control varieties into two categories, with  Yuelianggu belonging to  one group while the other eight  varieties to  another one. However,  clustering analysis based on SSR markers could divide  the tested moterials into two categories,  with one group including all Yuelianggu samples and the indica  control while another one only including  the japonica   control. Analysis of molecular variance （AMVOA）  revealed that  the intervillage genetic variations of Yuelianggu consisted of 67.18% and  the intravillage genetic variation contisted of 37.82%. Yuelianggu samples collected from 11 villages could be divided into 5 populations, which were closely related to geographical distance between the villages. Genetic distances of Yuelianggu collected from adjacent villages were small while their genetic similarities were high correspondly. The special genetic variation of Yuelianggu  resulted from  the unique Hani’s terraced fields and its traditional culture for longterm. Yuelianggu can be used as a benefit gene resource to further exploit and utilize.

Key words: Hani’s terraced field, rice, genetic variation, Yuelianggu

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